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Pricing

 

All prices shown are for CU researchers (must use a speedtype for payment). For external users, please contact Amber Scott (Biof-ngs@colorado.edu) for our current External price list. All prices are subject to change, please contact Amber Scott for the most current prices.

 

Sequencing Services

NextSeq runs: Pool QC included in cost. High output typically yields ~400 million reads. Mid output typically yields ~130 million reads.

NextSeq 500 (all V2)Pricing unitInternal CU PriceExternal Price
High output 75 cycle (1x75 or 2x37 PE) Run $2150.00 $2500.00
High output 150 cycle (1x150 or 2x75 PE) Run $3500.00 $4725.00
High output 300 cycle (2x150 PE) Run $5500.00 $7428.00
Mid output 150 cycle (1x150 or 2x75PE) Run $1600.00 $2160.00
Mid output 300 cycle (2x150 PE) Run $2400.00 $3240.00

MiSeq runs: Pool QC included in cost. V2 typically yields ~12-15 million reads. V3 typically yields ~22-25 million reads (including PhiX reads).

MiSeqPricing UnitInternal CU PriceExternal Price
V2 50 cycle kit (1x50 or 2x25 PE) Run $1300.00 $1755.00
V2 300 cycle kit (1x300 or 2x150 PE) Run $1625.00 $2193.00
V2 500 cycle kit (2x250 PE) Run $1750.00 $2362.00
V3 150 cycle kit (1x150 or 2x75 PE) Run $1450.00 $1957.50
V3 600 cycle kit (2x300 PE) Run $2150.00 $2902.50

MiSeq runs are priced per run but can be multiplexed within that sample.

 

Library Prep Services

DNA and RNA Library Prep: Library Prep includes all associated QC and pooling costs. Quantity discounts available  for >24 or >96 samples. Other kits and preps may be available, please inquire. 

DNA Library PrepPricing UnitInternal CU PriceExternal Price
Nextera XT DNA (small genomes/amplicons) Library $125.00 $187.50
KAPA HyperPrep or HyperPlus (PCR free or with PCR) Library $150.00 $225.00
RNA Library PrepPricing UnitInternal CU PriceExternal Price
KAPA HyperPrep mRNA (polyA enrichment) Library $185.00 $277.50
KAPA HyperPrep with RiboErase (rRNA depletion) Library $265.00 $350.00

  

Automated Services

Automated Library Prep: Automation is provided on our Eppendorf epMotion 5075TMX system. Automated Library Prep includes all associated QC and pooling costs. Unless noted otherwise, all prices are for sets of 8 samples (a column in a 96-well plate). The first column includes setup fees.

Automated Library PrepInternal CU PriceExternal Price
First 8 Additional 8 First 8 Additional 8
Nextera XT DNA, 0.5X reaction volume $600.00 $300.00 $825.00 $450.00
Nextera XT indexing PCR (amplicons) $175.00 $75.00 $202.50 $82.50
Other Automation
AmpureXP Bead Cleanup $135.00 $55.00 $202.50 $82.50
Sample quantification and pooling $50.00 $20.00 $75.00 $30.00

 

Other services

Other services: Include assays and instrumentation at the facility. Other services and quantity discounts may be available- please inquire. 

Other ServicesPricing UnitInternal CU PriceExternal Price
Sample Qubit (DNA or RNA) Sample $2.50 $3.75
TapeStation (Depends on assay chosen) Sample $12.50 $18.75

Sample qPCR (may take up to 5 business days)

Expedited qPCR

Sample

$45.00

$125.00

$67.50

$187.50

qPCR quantification  Plate $235.00 $352.50
BluePippin size selection Lane $35.00 $35.00
Covaris shearing Sample $35.00 $52.50

QuantStudio 6 qPCR (self-service)

(assisted service)

Plate

$15.00

$55.00

$22.50

$82.50

Other services prices include assays and instrumentation at the facility.

Other services may be available, please contact Amber Scott for a consultation, Biof-ngs@colorado.edu

 

Printable Services and Pricing Information can be found here

For Terms and Conditions, visit:

https://www.colorado.edu/ocg/manage-awards/service-activities/standard-service-quotation-terms-conditions

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