Molecular Devices ImageXpress

The Molecular Devices ImageXpress is a fully enclosed widefield microscope that is ideal for long-term imaging of multiwell plates with full environmental control. The system is equipped with 4 objectives and can house up to five different filter cubes which allows significant imaging versatility. 

Rules of Use:

1. The maximum duration of a reservation from 9:00am – 5:00pm is 3 hours, except for on Mondays and Fridays (please see #3). If you need more than 3 hours, like for an overnight experiment, please start your session after 4:00pm.
2. If you run an overnight experiment, you are not allowed to reserve a session during peak hours (9:00am - 5:00pm) the following day.  Please end your overnight session by 9:00am the following morning.
3. In general, please keep your Monday and Friday reservations to 3 hours. If you would like to do an extremely long experiment, you are allowed to start this on Friday at 9:00am and continue until Tuesday morning at 9:00am.
4. The microscope can be reserved for any amount of time starting at 4:00pm each afternoon and all day Saturday and Sunday (and Monday and Friday as outlined above).
5. University holidays and cancellations (i.e. snow days) will be treated like a Saturday or a Sunday.

• Light Source: Sola Light Engine.

• Objectives (Click here for more detailed information from Nikon):

  Position	Mag	NA	Immersion	Working Distance (µm)	Characteristics
  1	4x	0.13	Air	17000	UPlan FL N
  2	10x	0.3	Air	10000	UPlan FL N Ph1
  3	20x	0.5	Air	2100	UPlan FL N Ph1
  4	40x	0.75	Air	510	UPlan FL N2 Ph2

• Filter Cubes:

  Name	Excitation	Mirror	Emission	Fluorophores
  Dapi				Dapi Hoescht
  CFP				CFP Cerulean
  GFP				GFP FITC Alexa488
  YFP				YFP Citrene
  TRITC				TRITC Texas Red
  RFP				RFP
  mCherry				mCherry
  Cy3				TRITC Alexa568
  Cy5.5				Alexa647 Draq5
  CFP/YFP FRET				CFP/YFP FRET
  GFP/RFP FRET				GFP/RFP FRET

• Compatible Multiwell plate formats: 6, 12, 24, 48, 96, 384
• Environmental Control: CO2, Temperature, Humidity
• Camera: PCO sCMOS
• Key Specs:

  Active Pixels
  Pixel Size	6.45 x 6.45 µm
  Image Area pixel well depth
  Maximum Readout Rate
  Maximum Frame Rate
  Read Noise
  Maximum Cooling