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Molecular Devices ImageXpress

The Molecular Devices ImageXpress is a fully enclosed widefield microscope that is ideal for long-term imaging of multiwell plates with full environmental control. The system is equipped with 4 objectives and can house up to five different filter cubes which allows significant imaging versatility. 

Rules of Use:

  1. The maximum duration of a reservation from 9:00am – 5:00pm is 3 hours, except for on Mondays and Fridays (please see #3). If you need more than 3 hours, like for an overnight experiment, please start your session after 4:00pm.
  2. If you run an overnight experiment, you are not allowed to reserve a session during peak hours (9:00am - 5:00pm) the following day.  Please end your overnight session by 9:00am the following morning.
  3. In general, please keep your Monday and Friday reservations to 3 hours. If you would like to do an extremely long experiment, you are allowed to start this on Friday at 9:00am and continue until Tuesday morning at 9:00am.
  4. The microscope can be reserved for any amount of time starting at 4:00pm each afternoon and all day Saturday and Sunday (and Monday and Friday as outlined above).
  5. University holidays and cancellations (i.e. snow days) will be treated like a Saturday or a Sunday.
  • Objectives (Click here for more detailed information from Nikon):
    PositionMagNAImmersionWorking Distance (µm)Characteristics
    1 4x 0.13 Air 17000 UPlan FL N
    2 10x 0.3 Air 10000 UPlan FL N Ph1
    3 20x 0.5 Air 2100 UPlan FL N Ph1
    4 40x 0.75 Air 510 UPlan FL N2 Ph2
  • Filter Cubes:
    NameExcitationMirrorEmissionFluorophores
    Dapi Dapi
    Hoescht
    CFP CFP
    Cerulean
    GFP GFP
    FITC
    Alexa488
    YFP YFP
    Citrene
    TRITC TRITC
    Texas Red
    RFP RFP
    mCherry mCherry
    Cy3 TRITC
    Alexa568
    Cy5.5 Alexa647
    Draq5
    CFP/YFP FRET CFP/YFP FRET
    GFP/RFP FRET GFP/RFP FRET
  • Compatible Multiwell plate formats: 6, 12, 24, 48, 96, 384
  • Environmental Control: CO2, Temperature, Humidity
  • Camera: PCO sCMOS
  • Key Specs:
    Active Pixels
    Pixel Size 6.45 x 6.45 µm
    Image Area pixel well depth
    Maximum Readout Rate
    Maximum Frame Rate
    Read Noise
    Maximum Cooling

 

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