Major Equipment in the
BioFrontiers Advanced Light Microscopy Core
Director: Joe Dragavon, PhD
The BioFrontiers Advanced Light Microscopy Core is equipped with the following microscopes (click here to download):
Nikon A1R Laser Scanning Confocal and TIRF Inverted Microscope |
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Lasers: |
405, 458, 476, 488, 514, 561, 638 |
Detectors: |
4 independent PMTs |
Widefield light source |
Sola Light Engine |
Motorized XYZ Stage: |
Yes |
Piezo z insert: |
Yes |
Nikon Perfect Focus System: |
Yes |
Environmental Chamber: |
O2, CO2, Temperature, and Humidity control |
Camera: |
Andor Ixon3 897 EMCCD |
TIRF: |
Fully motorized |
Nikon N-STORM and TIRF Inverted Microscope |
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Lasers: |
405, 488, 561, 640 |
Cameras: |
2x Andor Ixon Ultra 897 EMCCD |
|
1x Hamamatsu ORCA Flash4.0 sCMOS |
Widefield light source: |
Excelitas X-Cite 120LED |
Motorized XYZ Stage: |
Yes |
Piezo z insert: |
Yes |
Nikon Perfect Focus System: |
Yes |
Environmental Chamber: |
O2, CO2, Temperature, and Humidity control |
TIRF: |
Fully motorized |
Molecular Devices ImageXpress Plate Scanner |
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Filter Cubes: |
Dapi, CFP, GFP, YFP, RFP, TRITC, mCherry |
|
CFP/YFP FRET, GFP/RFP FRET, Cy3, Cy5.5 |
Camera: |
PCO sCMOS |
Widefield Light Source: |
Sola Light Engine |
Motorized XYZ stage: |
Yes |
Near-infrared autofocus system: |
Yes |
Environmental chamber: |
CO2, Temperature, and Humidity control |
Capable of imaging multiwell plates: |
6, 12, 24, 96, 384 |
Olympus IX-81 Inverted Widefield Microscope |
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Light Source: |
Prior Lumen 200 Metal-Halide lamp |
Filter Cubes: |
Dapi, FITC, TRITC |
Camera: |
Hamamatsu ORCA-ER C4742-80 |
Motorized XYZ stage: |
Yes |
Piezo z: |
Yes |
Environmental chamber |
CO2, Temperature, and Humidity control |
Nikon Te-2000 Widefield |
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Light Source: |
HBO Lamp |
Camera: |
Nikon Digital Sight DS-Qi1Mc |
External Excitation Filter Wheel: |
10 positions |
External Emission Filter Wheel: |
10 Positions |
Motorized XY stage: |
Yes |
Nikon Spinning Disc Confocal |
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Lasers: |
405, 445, 488, 514, 561, 594, 647 |
Camera: |
2x Andor Ultra 888 EMCCD, 1k x 1k |
Spinning Disc: |
Yokogawa CSUX-A1 |
Widefield Light Source: |
HBO Arc Lamp |
Motorized XYZ stage: |
Yes |
Piezo z insert: |
Yes |
Nikon Perfect Focus System: |
Yes |
Environmental Chamber: |
CO2, Temperature, and Humidity control |
Analysis Workstation |
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Hardware |
Graphics Card: |
GeForce GTX 690 for |
|
CUDA enabled GPU parallel processing |
Solid State Drives: |
Intel 520 Series |
Hard Drive: |
Seagate 7200rpm |
Intel Quad Core i7 |
Yes |
RAM: |
24 GB of Kingston 2400 MHz DDR3 RAM |
Motherboard: |
Asus z87 Deluxe |
|
|
Software |
Matlab: |
R2013b |
Imaris: |
8.0 |
Nikon Elements: |
4.3 |
ImageJ/Fiji: |
Yes |
ICY: |
Yes |
Cell Profiler |
Yes |