Major Equipment in the
BioFrontiers Advanced Light Microscopy Core
Director: Joe Dragavon, PhD
The BioFrontiers Advanced Light Microscopy Core is equipped with the following microscopes (click here to download):
| Nikon A1R Laser Scanning Confocal and TIRF Inverted Microscope |
|---|
| Lasers: |
405, 458, 476, 488, 514, 561, 638 |
| Detectors: |
4 independent PMTs |
| Widefield light source |
Sola Light Engine |
| Motorized XYZ Stage: |
Yes |
| Piezo z insert: |
Yes |
| Nikon Perfect Focus System: |
Yes |
| Environmental Chamber: |
O2, CO2, Temperature, and Humidity control |
| Camera: |
Andor Ixon3 897 EMCCD |
| TIRF: |
Fully motorized |
| Nikon N-STORM and TIRF Inverted Microscope |
|---|
| Lasers: |
405, 488, 561, 640 |
| Cameras: |
2x Andor Ixon Ultra 897 EMCCD |
|
1x Hamamatsu ORCA Flash4.0 sCMOS |
| Widefield light source: |
Excelitas X-Cite 120LED |
| Motorized XYZ Stage: |
Yes |
| Piezo z insert: |
Yes |
| Nikon Perfect Focus System: |
Yes |
| Environmental Chamber: |
O2, CO2, Temperature, and Humidity control |
| TIRF: |
Fully motorized |
| Molecular Devices ImageXpress Plate Scanner |
|---|
| Filter Cubes: |
Dapi, CFP, GFP, YFP, RFP, TRITC, mCherry |
|
CFP/YFP FRET, GFP/RFP FRET, Cy3, Cy5.5 |
| Camera: |
PCO sCMOS |
| Widefield Light Source: |
Sola Light Engine |
| Motorized XYZ stage: |
Yes |
| Near-infrared autofocus system: |
Yes |
| Environmental chamber: |
CO2, Temperature, and Humidity control |
| Capable of imaging multiwell plates: |
6, 12, 24, 96, 384 |
| Olympus IX-81 Inverted Widefield Microscope |
|---|
| Light Source: |
Prior Lumen 200 Metal-Halide lamp |
| Filter Cubes: |
Dapi, FITC, TRITC |
| Camera: |
Hamamatsu ORCA-ER C4742-80 |
| Motorized XYZ stage: |
Yes |
| Piezo z: |
Yes |
| Environmental chamber |
CO2, Temperature, and Humidity control |
| Nikon Te-2000 Widefield |
|---|
| Light Source: |
HBO Lamp |
| Camera: |
Nikon Digital Sight DS-Qi1Mc |
| External Excitation Filter Wheel: |
10 positions |
| External Emission Filter Wheel: |
10 Positions |
| Motorized XY stage: |
Yes |
| Nikon Spinning Disc Confocal |
|---|
| Lasers: |
405, 445, 488, 514, 561, 594, 647 |
| Camera: |
2x Andor Ultra 888 EMCCD, 1k x 1k |
| Spinning Disc: |
Yokogawa CSUX-A1 |
| Widefield Light Source: |
HBO Arc Lamp |
| Motorized XYZ stage: |
Yes |
| Piezo z insert: |
Yes |
| Nikon Perfect Focus System: |
Yes |
| Environmental Chamber: |
CO2, Temperature, and Humidity control |
| Analysis Workstation |
|---|
| Hardware |
| Graphics Card: |
GeForce GTX 690 for |
|
CUDA enabled GPU parallel processing |
| Solid State Drives: |
Intel 520 Series |
| Hard Drive: |
Seagate 7200rpm |
| Intel Quad Core i7 |
Yes |
| RAM: |
24 GB of Kingston 2400 MHz DDR3 RAM |
| Motherboard: |
Asus z87 Deluxe |
|
|
| Software |
| Matlab: |
R2013b |
| Imaris: |
8.0 |
| Nikon Elements: |
4.3 |
| ImageJ/Fiji: |
Yes |
| ICY: |
Yes |
| Cell Profiler |
Yes |
