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CU-Boulder Core Facilities and Service Centers

 

Below is a list of many other Core Facilities and Service Centers that are present on the CU-Boulder campus.  These entities are here to assist the Boulder scientific community with their research endeavors.  Each is staffed with qualified professionals that are capable of greatly accelerating your work.

Please contact if you would like to modify any information provided below or to suggest another Core Facility or Service Center to add to this list.

Click here to download the pamphlet from the 2015 CU-Boulder Core Facility and Service Center Open House.

Click here to download all of the presentations from the Open House

Genomics

BioFrontiers Next-Gen Sequencing Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Jamie Kershner
  • 303-735-1255
  • jamie.prior@colorado.edu                
  • BioFrontiers Institute
  • JSCBB C1B76
https://bficores.colorado.edu/sequencing-lab
The sequencing facility has several Illumina sequencer including 2 MiSeqs and a HiSeq 2000. We also support sequencing library construction, either as a provided service or provide access to instrumentation for library QA/QC. We have several pieces of equipment available to users including a Qubit, Bioanalyzer,  and QuantStudio 6 qPCR. Consultations with the Director (Jamie Kershner) are encouraged at any point during a project from early planning stages to sample submission for sequencing. External projects both within academia and biotech industry are welcome. Please see our website for a complete listing of our equipment and services.

 

Functional Genomics Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Molishree Joshi
  • 303-492-7529
  • funcgenfac@colorado.edu
  • MCDB          
  • Porter B140
https://functionalgenomics.colorado.edu/index/html
The FGF is a non-profit core facility that aims to promote scientific research in the area of functional genomics in Colorado. Over time, our core facility has become one of the largest repositories of functional genomics tools in the world. We administer a human shRNA collection (176,283 clones targeting >22,000 unique genes), a mouse shRNA collection (138,538 clones targeting >21,000 unique genes), a human Open Reading Frame (ORF) library (15,744 ORFs corresponding to 13,082 unique human genes), diverse gene knockout pooled CRISPR libraries (6 gRNAs per gene to inactive ~18,000 human genes and ~15,000 mouse genes) and a transactivating CRISPR library to induce expression of ~18,000 human promoters. We continue to identify and acquire/add key resources to advance research in the area of Functional Genomics. We also provide assistance with genome-wide screen design and analysis on a per need basis through phone, email and in person communication.


Microscopy

BioFrontiers Advanced Light Microscopy Core Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Joe Dragavon
  • 303-735-6988
  • joseph.dragavon@colorado.edu
  • BioFrontiers Institute
  • JSCBB C350
https://bficores.colorado.edu/imaging-facility
Multiple imaging technologies are available within the BioFrontiers Advanced Light Microscopy Core, including widefield (Zeiss Axiovert 200M, Nikon Te-2000, Olympus IX-81), spinning disc confocal (Nikon TiE with a Yokogawa CSUX A1), laser scanning confocal (Nikon A1R TiE), plate scanning (Molecular Devices ImageXpress), and super resolution (Nikon N-STORM). Additionally there is a dedicated platform for image analysis that includes Nikon Elements, Imaris, Matlab, ImageJ/Fiji, ICY and Cell Profiler.


Light Microscopy Core Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Jolien Tyler
  • 303-492-5955
  • jolien.tyler@colorado.edu
  • MCDB
  • Porter B047A
http://mcdb.colorado.edu/facilities/lmcf
The LMCF houses a wide range of widefield and confocal fluorescence light microscopes. In addition to our Nikon spinning disk and Zeiss laser scanning confocals, we have a combination Nikon A1 laser scanning confocal and SIM structured illumination super-resolution microscope. SIM super-resolution is amenable to a wide range of sample types and can achieve a two-fold resolution improvement over conventional microscopy techniques.


Boulder Electron Microscopy Services (2015 CU-Boulder Core Facility and Open House presentation)

  • Tom Giddings
  • 303-492-8402
  • BoulderEM@colorado.edu
  • MCDB
http://mcdb.colorado.edu/facilities/ems/
The CU-Boulder Electron Microscopy Service provides access to a full range of transmission electron microscopy imaging services including conventional TEM, electron tomography and cryo-electron microscopy. The facility houses three conventional transmission electron microscopes (Philips CM10, Philips CM100 and Tecnai Spirit BioTwin) and two intermediate voltage transmission electron microscopes (200kV FEI Tecnai F20 and 300kV FEI Tecnai F30). Supported specimen preparation services include negative staining, chemical fixation, high pressure (Wohlwend Compact 02 HPF) and plunge freezing (FEI Vitrobot), freeze-substitution, plastic- and cryo-sectioning, immuno-labeling, freeze-fracture and low-angle rotary shadowing.


Electron Microprobe Laboratory (2015 CU-Boulder Core Facility and Open House presentation)

  • Julien Allaz
  • julien.allaz@colorado.edu
  • Geological Sciences
  • Benson 125B
http://geode.colorado.edu/~jallaz/index.php?page=microprobe
An electron microprobe is an electron microscope designed for the non-destructive X-ray microanalysis and imaging of solid materials. It is capable of high spatial resolution (1 µm beam size) and relatively high analytical sensitivity. The analytical facilities' JEOL JXA-8600 can acquire digital secondary-electron and backscattered-electron images as well as elemental x-ray maps. It is equipped with 4 wavelength-dispersive spectrometers and an energy-dispersive spectrometer. Most of the periodic table can in principle be analyzed (Be through U), subject to several important considerations. In early 2016, a new instrument will be installed (JEOL JXA-8230), which will considerably increase our analytical capabilities, notably for trace elements analysis (down to 10 ppm), and spatial resolution (0.7-0.2 µm). The new instrument will include 5 spectrometers, a solid-state EDS detector capable of light element detection, and a panchromatic CL detector. We are open to internal and external researchers, as also to industry/private users! Laboratory visits can be arranged.


Freeze-Fracture Transmission Electron Microscopy

  • Mike Tuchband
  • 303-492-3585
  • michael.tuchband@colorado.edu
  • Physics
  • Duane E2B32
https://smrc.colorado.edu/facilities/FFTEM_system.html
Freeze-fracture transmission electron microscopy (FFTEM) is a powerful replica-based technique for imaging the nanometer-scale structure of soft materials, revealing features as small as a few nanometers. We use FFTEM to study the bulk and interfacial structure of equilibrium and non-equilibrium states of liquid crystals, nanocolloids, and other nanostructured soft materials.


Flow Cytometry

Flow Cytometry Shared Core

  • Theresa Nahreini
  • 303-492-3585
  • nahreini@colorado.edu
  • Chem Biochem
  • JSCBB C355
High-performance analysis and sorting of mammalian cells, bacterial, yeast, algae and nanoparticles. We currently offer 488 and 561 nm lasers with plans for a 445 nm excitation source. Up to 4-way sorting options are available.

 

Flow Cytometry Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Yuming Han
  • 303-492-2724
  • yhan@colorado.edu
  • MCDB
  • Porter B059, B051
http://mcdb.colorado.edu/facilities/flow_cyt/
With a CyAN ADP Analyzer and a MoFlo XDP Cell Sorter, the MCDB Flow Cytometry Facility offers state-of-art cell sorting and analysis service to the growing user groups in Boulder biomedical research community. The Cyan ADP Analyzer accommodates a diverse range of applications up to 11 parameters and 9 colors for analyzing mammalian cells, yeast, bacteria and other cells.  MoFlo XDP Cell Sorter, also with 3 lasers and 9-color capacity, can perform 4-way sorting of various mammalian cells. It enables configuration modularity to meet the diverse sorting applications including those for 96-well plates.

 

Spectroscopy

Central Analytical Mass Spectrometry & Proteomics Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Jeremy Balsbaugh
  • 303-735-4019
  • jeremy.balsbaugh@colorado.edu
  • Chem Biochem
  • JSCBB C1B90
http://chem.colorado.edu/massspec/
We specialize in proteomics-based mass spectrometry analyses that implement high resolution liquid chromatography separations and instruments capable of high resolution, accurate mass determinations.  We routinely provide bioinformatics data processing for all datasets.  We also provide additional analyses including, but not limited to, the identification and quantitation of small organic molecules, oligonucleotides, peptides, proteins and polymers using a variety of instruments and mass spectrometry-based methods.


NMR Spectroscopy Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Richard Shoemaker
  • richard.shoemaker@colorado.edu
  • Chem Biochem
  • Cristol CHEM-02, JSCBB C1B82
http://chemnmr.colorado.edu
NMR Spectroscopy for small molecules, and materials. Field/Frequencies from 300MHz to 500MHz, with solid-state (MAS) NMR capabilities at 400MHz. Hands-on operation is available for most user-groups, as well as service spectroscopy when requested and as appropriate.


High-Field NMR Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Geoff Armstrong
  • geoffrey.armstrong@colorado.edu
  • Chem Biochem
  • CU Boulder CINC Building
  • CU Anschutz Medical Campus
http://cunmr800.colorado.edu
800 and 900 MHz NMR Spectrometers with Cryogenically cooled probes. Structure determination with Rosetta.


Raman Microspectroscopy Lab (2015 CU-Boulder Core Facility and Open House presentation)

  • Eric Ellison
  • 303-492-7025
  • eric.ellison@colorado.edu
  • Geological Sciences
  • Benson 125A
https://spot.colorado.edu/~templeta/Templeton_Lab/Raman_Lab.html
The Raman Microspectroscopy Lab is equipped with a Horiba LabRAM HR Evolution Raman microscope-spectrometer capable of fast, non-destructive chemical imaging and vibrational characterization of a diversity of material types, including minerals (thin sections & powders), biological samples, fluids, dissolved gases, and much more. Technical assistance is always available, both during and after data collection. We also provide access to state-of-the-art multivariate statistical/chemometric software for analysis of hyperspectral Raman image data.


Organic and Isotope Geochemistry Laboratory (2015 CU-Boulder Core Facility and Open House presentation)

  • Julio Sepúlveda
  • 303-735-6877
  • jsepulveda@colorado.edu
  • Geological Sciences
  • Institute of Arctic and Alpine Research (INSTAAR)
  • SEEC
https://instaar.colorado.edu/people/julio-sepulveda/
The Organic and Isotope Geochemistry Laboratory—expected to open its doors in Spring-Summer 2016—is a new state-of-the-art analytical facility in the Sustainability, Energy and Environment Complex (SEEC). The lab will be equipped with instrumentation for gas and liquid chromatography-mass spectrometry (GC-QQQ-MS and HPLC-QTOF), in addition to gas chromatography-combustion-isotope ratio-mass spectrometry (GC-c-ir-MS). The array of instruments will allow the chemical and isotopic characterization of a wide range of organic molecules in modern and ancient environments. The laboratory will serve as a new analytical platform for research fields such as biogeochemistry, geobiology, astrobiology, environmental microbiology, environmental pollution, energy, archaeology, and paleoceanography and paleoclimatology, among others.


CU Boulder Earth Systems Stabel Isotope Laboratory (CUBES-SIL) (2015 CU-Boulder Core Facility and Open House presentation)

  • Kathryn Snell
  • 303-492-1461
  • kathryn.snell@colorado.edu
  • Geological Sciences
  • Benson 345
Brief description: When completed, this facility will house 2 magnetic sector, gas-source stable isotope ratio mass spectrometers and associated peripherals. One instrument will be used to measure low abundance, multiply-substituted isotopologues of CO2 and other high precision applications. The second instrument will be capable of analyzing C, H, N, O and S isotopes from a range of gaseous, liquid and solid materials, with applications in a broad range of disciplines including geology, ecology, anthropology, microbiology and forensics.


Computation

BioFrontiers Computing Core (2015 CU-Boulder Core Facility and Open House presentation)

  • Dan Timmons
  • 303-492-7579
  • dan.timmons@colorado.edu
  • BioFrontiers Institute
  • JSCBB
https://bficores.colorado.edu/biofrontiers-it
The BFI Computing cores mission provides high-powered computing services to the BioFrontiers core faculty, BioFrontiers core sequencing and imaging facilities and limited services to BioFrontiers collaborators.


X-Ray and High Throughput Screening

Macromolecular X-Ray Crystallography (2015 CU-Boulder Core Facility and Open House presentation)

  • Dave McKay
  • david.mckay@colorado.edu
  • Chem Biochem
  • JSCBB C1B51
https://biofrontiers.colorado.edu/core-facilities/x-ray-crystallography
For macromolecular crystallography: robotic crystallization dropsetter; crystallization imagers; in-house data collection systems with cryocooling; hardware for remote data collection at synchrotrons


X-Ray Diffraction Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Rayshan Visvanathan
  • rayshan.visvanathan@colorado.edu
  • Physics
  • Duane C232
https://smrc.colorado.edu/facilities/xrd_waxs.html
A custom-built wide angle x-ray scattering (WAXS) system with a Huber 4-circle goniometer capable of accommodating large sample chambers (such as temperature-controlled ovens), this diffractometer is used routinely for structural characterization of liquid crystalline, polymeric and biological materials, x-ray reflectivity measurements on thin films, and high-resolution powder diffraction of polycrystalline materials. We are currently in the process of acquiring a custom built, modular small-angle x-ray scattering (SAXS) diffractometer with a high-brilliance x-ray source and a high-resolution area detector with single-photon sensitivity, and with a beam path that can be reconfigured for SAXS, WAXS, and grazing-incidence (GISAXS) measurements. We anticipate that this system will come on line near the end of September 2015.


High-Throughput Screening Core Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Wei Wang
  • 303-492-9123
  • wang.wei@colorado.edu
  • Chem Biochem
  • Cristol
http://chem.colorado.edu/cub_hts
The HTS Core Facility is a non-profit facility, which aims to promote chemical biology research and drug discovery at CU-Boulder and the surrounding areas. Our facility is equipped with state-of-the-art instruments for both high-throughput and high-content biochemical and cell-based assays, including live-cell assays. We offer assistance on all steps involved in typical drug-discovery processes: (1) Assay development, optimization and validation, (2) High-throughput screening, (3) Data analysis, validation and lead identification, and (4) SAR studies and large-scale production. In addition, we offer access to the complete package of Schrodinger Suite for in silico screening and post-screen cheminformatic analysis.


General Instrumentation

Biochemistry Shared Instruments Core (2015 CU-Boulder Core Facility and Open House presentation)

  • Annette Erbse
  • 303-492-0528
  • erbse@colorado.edu
  • Chem Biochem
  • JSCBB
 https://chem.colorado.edu/biochemcore
Investigators of the Biochemistry Division of the Department for Chemistry and Biochemistry at the University of Colorado at Boulder have joined forces and placed some of their State-of- the-Art instrumentation in the newly established instrument core to facilitate the sharing of instrumentation and analysis capabilities. The instrument core gives researchers in the department but also from the broader CU community and from outside the university access to a multitude of state of the art instruments and techniques including EPR, CD and fluorescence spectroscopy, DLS, Sec-MALS and ITC. For measurements of fast reaction kinetics it offers stopped-flow and chemical quench flow techniques. In addition it houses a variety of additional equipment, such as imaging systems, centrifuges, scintillation counter, sonicator and more.


Clean Room and Fabrication

JILA Clean Room and Metrology

  • David Alchenberger
  • 303-492-2389
  • alchenbd@colorado.edu
  • JILA
  • JILA X131 and S105
https://jila.colorado.edu/scientific-support/keck-lab

Metrology Lab: Optical measurement and characterization instruments fabrication.

Clean Room: a suite of Class 1000 (ISO 6) and Class 100 (ISO 5) cleanroom bays. Though designed to support research at JILA, the facility is also available to other University of Colorado researchers and local industry on a space-available basis.


Chemical & Biological Engineering Instrument Shop (2015 CU-Boulder Core Facility and Open House presentation)

  • Dragan Mejic
  • dragan.mejic@colorado.edu
  • Chem Biochem
  • JSCBB Basement
Complete design and fabrication of mechanical and electronic devices including flow cells, high temperature and high-pressure reactors, electromechanical assemblies, custom microscope stages, heating and control circuits, and many more.


Electronics Design and Build Shop (2015 CU-Boulder Core Facility and Open House presentation)

  • Maria Toscano-Leary
  • Dana Huaschulz
  • maria.toscano@colorado.edu
  • dana.hauschulz@colorado.edu
  • Chem Biochem
  • JSCBB B1B01
We design and build electro/mechanical equipment. We also troubleshoot systems and repair lab equipment.


Colorado Nanofabrication Laboratory & the Nanomaterials Characterization Facility (2015 CU-Boulder Core Facility and Open House presentation)

  • Tomoko Borsa
  • borsa@colorado.edu
  • ECEE
  • CNL: ECEE 2nd Floor
  • NCF: DLC 2nd Floor
https://cnl.colorado.edu

CNL: Fabrication tools including lithography, thin film deposition and etching.

NCF: Characterization tools including FIB (TEM sample preparation), FESEM, SEMs, AFM and EM sample preparation capability









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