FastQCFastQC Report
Fri 3 Jun 2016
OM28_S7_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameOM28_S7_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981957
Sequences flagged as poor quality0
Sequence length151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG5602.4901965E-916.8135151
GTATTGG10750.014.8223611
GTCTTAG4151.7957824E-413.9619181
GTATTAA6051.8264527E-613.1686271
GTACTTA3850.001616251513.1686276
TAGTAGA7751.2680175E-813.0836692
TACTGGA14200.012.751312
GTTAGGG7551.2711098E-712.4709512
CCAAGAG11209.094947E-1212.28679853
GTCCTAC5952.2250715E-512.1726811
GAATAGG7801.9423715E-712.071241
TATACCG11451.4551915E-1112.0185285
GTATGAA8454.2391548E-811.9998131
TCTCGGG35300.011.941533145
GTATAGG6102.8713699E-511.8733521
TATAATG10003.0104275E-911.5883922
CTAGCAC5008.6489617E-411.5883923
CTTGGAC7601.8586015E-611.4359133
GTCATAG8908.71205E-811.3930821
TCTTAAG10858.931238E-1011.3480792