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Olympus IX-81

The Olympus IX-81 is a widefield microscope equipped with 6 objectives and 3 filter cubes. This system is simple to use and your samples can be quickly imaged. It is equipped with an ORCA-ER high resolution digital camera. The motorized stage allows for the automated scanning of your sample slides.

 

Olympus IX-81 user manual coming soon.

 

  • Objectives (Click here for more detailed information from Olympus):
    PositionMagNAImmersionWorking Distance (µm)Characteristics
    1 4x 0.13 Air 17000 UPlan FL N
    2 10x 0.3 Air 10000 UPlan FL N Ph1
    3 20x 0.5 Air 2100 UPlan FL N Ph1
    4 40x 0.75 Air 510 UPlan FL N2 Ph2
    5 60x 1.4 Oil 150 Plan Apo
    6 100x 1.35 Oil 100 UPlan Apo Iris

 

  • Filter Cubes:
    NameExcitation (nmMirror (nm)Emission (nm)Fluorophore
    DAPI 352 - 402 409LP 417 - 477 Dapi
    Hoescht
    Alexa405
    CFP
    FITC 464 - 500 506LP 516 - 556 FITC
    Alexa488
    GFP
    TRITC 532 - 544 562LP 573 - 613 TRITC
    Alexa568
    mCherry

 

  • Camera: Hamamatsu ORCA-ER C4742-80-12AG
  • Key Specs:
    Active Pixels 1344 (H) x 1024 (W)
    Pixel Size 6.45 x 6.45 µm
    Image Area pixel well depth 18,000 e-
    Maximum Readout Rate 14.75MHz
    Maximum Frame Rate 8.8 - 41 fps
    Read Noise < 1 e- with EM gain
    Maximum Cooling -30 ˚C

 

  • Pixel Size on Image: (Click here for a nice overview)
    Objective
    Lens Magnification EMCCD Pixel Size 4x 10x 20x 40x 60x 100x
    1 6450 x 6450 1612.5 645 322.5 161.25 107.5 64.5
    1.6 6450 x 6450 1007.81 403.13 201.56 100.78 67.19 40.31
  • Image pixel size = (Camera pixel size x bin factor)/(objective magnification x lens magnification x C mount magnification).
  • Pay attention to your units! The units in the table are nm.
  • Most often the lens magnification and C mount magnification = 1.

 

 

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