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Equipment Summary

Major Equipment in the

BioFrontiers Advanced Light Microscopy Core

Director: Joe Dragavon, PhD

 

The BioFrontiers Advanced Light Microscopy Core is equipped with the following microscopes (click here to download):

Nikon A1R Laser Scanning Confocal and TIRF Inverted Microscope
Lasers: 405, 458, 476, 488, 514, 561, 638
Detectors: 4 independent PMTs
Widefield light source Sola Light Engine
Motorized XYZ Stage: Yes
Piezo z insert: Yes
Nikon Perfect Focus System: Yes
Environmental Chamber: O2, CO2, Temperature, and Humidity control
Camera: Andor Ixon3 897 EMCCD
TIRF: Fully motorized
Nikon N-STORM and TIRF Inverted Microscope
Lasers: 405, 488, 561, 640
Cameras: 2x Andor Ixon Ultra 897 EMCCD
1x Hamamatsu ORCA Flash4.0 sCMOS
Widefield light source: Excelitas X-Cite 120LED
Motorized XYZ Stage: Yes
Piezo z insert: Yes
Nikon Perfect Focus System: Yes
Environmental Chamber: O2, CO2, Temperature, and Humidity control
TIRF: Fully motorized
Molecular Devices ImageXpress Plate Scanner
Filter Cubes: Dapi, CFP, GFP, YFP, RFP, TRITC, mCherry
CFP/YFP FRET, GFP/RFP FRET, Cy3, Cy5.5
Camera: PCO sCMOS
Widefield Light Source: Sola Light Engine
Motorized XYZ stage: Yes
Near-infrared autofocus system: Yes
Environmental chamber: CO2, Temperature, and Humidity control
Capable of imaging multiwell plates: 6, 12, 24, 96, 384
Olympus IX-81 Inverted Widefield Microscope
Light Source: Prior Lumen 200 Metal-Halide lamp
Filter Cubes: Dapi, FITC, TRITC
Camera: Hamamatsu ORCA-ER C4742-80
Motorized XYZ stage: Yes
Piezo z: Yes
Environmental chamber CO2, Temperature, and Humidity control
Nikon Te-2000 Widefield
Light Source: HBO Lamp
Camera: Nikon Digital Sight DS-Qi1Mc
External Excitation Filter Wheel: 10 positions
External Emission Filter Wheel: 10 Positions
Motorized XY stage: Yes
Nikon Spinning Disc Confocal
Lasers: 405, 445, 488, 514, 561, 594, 647
Camera: 2x Andor Ultra 888 EMCCD, 1k x 1k
Spinning Disc: Yokogawa CSUX-A1
Widefield Light Source: HBO Arc Lamp
Motorized XYZ stage: Yes
Piezo z insert: Yes
Nikon Perfect Focus System: Yes
Environmental Chamber: CO2, Temperature, and Humidity control
Analysis Workstation
Hardware
Graphics Card: GeForce GTX 690 for
CUDA enabled GPU parallel processing
Solid State Drives: Intel 520 Series
Hard Drive: Seagate 7200rpm
Intel Quad Core i7 Yes
RAM: 24 GB of Kingston 2400 MHz DDR3 RAM
Motherboard: Asus z87 Deluxe
Software
Matlab: R2013b
Imaris: 8.0
Nikon Elements: 4.3
ImageJ/Fiji: Yes
ICY: Yes
Cell Profiler Yes
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