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Quiz: Immersion Media and Microscope Objectives

You must complete and pass the following quiz about immersion media and microscope objectives before you can be trained to independently use a microscope within the facility. The quiz includes 15 multiple choice questions, with the answers found within the text and videos found on the Immersion Media and Microscope Objectives page. If you cannot find the answers here, feel free to use Google or your favorite search engine (extra credit to those that use Ask Jeeves, negative credit to those that use Bing. Don't get me started on Yahoo!). You must get 100% correct to pass. If you do not get 100%, we will go over the quiz together before scheduling your training session.

  Oil Water Air (Nothing)
4x 0.20NA Plan Apo
10x 0.45NA Plan Apo
20x 0.75NA Plan Apo
40x 0.95NA Plan Apo
40x 1.30NA Oil S Fluor
60x 1.20NA WI Plan Apo
60x 1.40NA Oil Apo
100x 1.45NA Oil Plan Apo
100x 1.49NA Oil Plan Apo TIRF
Which equation correctly describes the resolving power of an objective?  



In the above equation, NA stands for:  



In the equation, NA = n*sin(a0), n = ?  



In the equation, NA = n*sin(a0), a0 = ?  



What is sin(90˚)?  




Why do you need to add immersion medium to some objectives?  



Typically, do objectives that use immersion media have more or less resolving power than air objectives?  



How many drops of oil or water do you add to the objective?  



Ideally, do you image through the cover slip or the microscope slide?  



What should you do if you add too much immersion medium?  



What do you use to remove immersion medium from an objective?  






To remove immersion medium from an objective you are supposed to:  



What do you do if you accidentally add the wrong immersion medium to an objective?  


What do you do in case you are unsure if whether your objective requires immersion medium?  





Did you use a search engine to help you for this quiz? If so, which one? (These are/were all real by the way)  









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